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"en": "Gucciardo, Loukovaara, Korhonen, Repo, Martins, Vihinen, Jokitalo, Lehti: The microenvironment of proliferative diabetic retinopathy supports lymphatic neovascularization",
"fi": "Gucciardo, Loukovaara, Korhonen, Repo, Martins, Vihinen, Jokitalo, Lehti: The microenvironment of proliferative diabetic retinopathy supports lymphatic neovascularization"
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"en": "<jats:title>Abstract</jats:title><jats:p>Proliferative diabetic retinopathy (PDR) is a major diabetic microvascular complication characterized by pathological angiogenesis. Several retinopathy animal models have been developed to study the disease mechanisms and putative targets. However, knowledge on the human proliferative disease remains incomplete, relying on steady‐state results from thin histological neovascular tissue sections and vitreous samples. New translational models are thus required to comprehensively understand the disease pathophysiology and develop improved therapeutic interventions. We describe here a clinically relevant model, whereby the native multicellular PDR landscape and neo(fibro)vascular processes can be analysed <jats:italic>ex vivo</jats:italic> and related to clinical data. As characterized by three‐dimensional whole‐mount immunofluorescence and electron microscopy, heterogeneity in patient‐derived PDR neovascular tissues included discontinuous capillaries coupled with aberrantly differentiated, lymphatic‐like and tortuous endothelia. Spatially confined apoptosis and proliferation coexisted with inflammatory cell infiltration and unique vascular islet formation. <jats:italic>Ex vivo</jats:italic>‐cultured explants retained multicellularity, islet patterning and capillary or fibrotic outgrowth in response to vitreoretinal factors. Strikingly, PDR neovascular tissues, whose matched vitreous samples enhanced lymphatic endothelial cell sprouting, contained lymphatic‐like capillaries <jats:italic>in vivo</jats:italic> and developed Prox1<jats:sup>+</jats:sup> capillaries and sprouts with lymphatic endothelial ultrastructures <jats:italic>ex vivo</jats:italic>. Among multiple vitreal components, vascular endothelial growth factor C was one factor found at lymphatic endothelium‐activating concentrations. These results indicate that the ischaemia‐induced and inflammation‐induced human PDR microenvironment supports pathological neolymphovascularization, providing a new concept regarding PDR mechanisms and targeting options. Copyright © 2018 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.</jats:p>",
"fi": "<jats:title>Abstract</jats:title><jats:p>Proliferative diabetic retinopathy (PDR) is a major diabetic microvascular complication characterized by pathological angiogenesis. Several retinopathy animal models have been developed to study the disease mechanisms and putative targets. However, knowledge on the human proliferative disease remains incomplete, relying on steady‐state results from thin histological neovascular tissue sections and vitreous samples. New translational models are thus required to comprehensively understand the disease pathophysiology and develop improved therapeutic interventions. We describe here a clinically relevant model, whereby the native multicellular PDR landscape and neo(fibro)vascular processes can be analysed <jats:italic>ex vivo</jats:italic> and related to clinical data. As characterized by three‐dimensional whole‐mount immunofluorescence and electron microscopy, heterogeneity in patient‐derived PDR neovascular tissues included discontinuous capillaries coupled with aberrantly differentiated, lymphatic‐like and tortuous endothelia. Spatially confined apoptosis and proliferation coexisted with inflammatory cell infiltration and unique vascular islet formation. <jats:italic>Ex vivo</jats:italic>‐cultured explants retained multicellularity, islet patterning and capillary or fibrotic outgrowth in response to vitreoretinal factors. Strikingly, PDR neovascular tissues, whose matched vitreous samples enhanced lymphatic endothelial cell sprouting, contained lymphatic‐like capillaries <jats:italic>in vivo</jats:italic> and developed Prox1<jats:sup>+</jats:sup> capillaries and sprouts with lymphatic endothelial ultrastructures <jats:italic>ex vivo</jats:italic>. Among multiple vitreal components, vascular endothelial growth factor C was one factor found at lymphatic endothelium‐activating concentrations. These results indicate that the ischaemia‐induced and inflammation‐induced human PDR microenvironment supports pathological neolymphovascularization, providing a new concept regarding PDR mechanisms and targeting options. Copyright © 2018 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.</jats:p>",
"und": "<jats:title>Abstract</jats:title><jats:p>Proliferative diabetic retinopathy (PDR) is a major diabetic microvascular complication characterized by pathological angiogenesis. Several retinopathy animal models have been developed to study the disease mechanisms and putative targets. However, knowledge on the human proliferative disease remains incomplete, relying on steady‐state results from thin histological neovascular tissue sections and vitreous samples. New translational models are thus required to comprehensively understand the disease pathophysiology and develop improved therapeutic interventions. We describe here a clinically relevant model, whereby the native multicellular PDR landscape and neo(fibro)vascular processes can be analysed <jats:italic>ex vivo</jats:italic> and related to clinical data. As characterized by three‐dimensional whole‐mount immunofluorescence and electron microscopy, heterogeneity in patient‐derived PDR neovascular tissues included discontinuous capillaries coupled with aberrantly differentiated, lymphatic‐like and tortuous endothelia. Spatially confined apoptosis and proliferation coexisted with inflammatory cell infiltration and unique vascular islet formation. <jats:italic>Ex vivo</jats:italic>‐cultured explants retained multicellularity, islet patterning and capillary or fibrotic outgrowth in response to vitreoretinal factors. Strikingly, PDR neovascular tissues, whose matched vitreous samples enhanced lymphatic endothelial cell sprouting, contained lymphatic‐like capillaries <jats:italic>in vivo</jats:italic> and developed Prox1<jats:sup>+</jats:sup> capillaries and sprouts with lymphatic endothelial ultrastructures <jats:italic>ex vivo</jats:italic>. Among multiple vitreal components, vascular endothelial growth factor C was one factor found at lymphatic endothelium‐activating concentrations. These results indicate that the ischaemia‐induced and inflammation‐induced human PDR microenvironment supports pathological neolymphovascularization, providing a new concept regarding PDR mechanisms and targeting options. Copyright © 2018 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.</jats:p>"
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"fi": "<jats:p>The climate change -driven increase in temperature is occurring rapidly and decreasing the predictability of seasonal rhythms at high latitudes. It is therefore urgent to understand how a change in the relationship between the photoperiod and temperature can affect ectotherms in these environments. We tested whether temperature affects daily rhythms of transcription in a cold-adapted salmonid using high-throughput RNA-sequencing. Arctic char (Salvelinus alpinus) from a subarctic population were reared at a high and a low temperature (15°C and 8°C) for one month under natural, decreasing day length during late summer. Liver transcriptomes were compared between samples collected in the middle and towards the end of the light period and in the middle of the dark period. Daily variation in transcription was lower in fish from the low temperature compared to strong daily variation in warm-acclimated fish, suggesting cold temperatures dampen the cycling of transcriptional rhythms under a simultaneously decreasing day length. Different circadian clock genes had divergent expression patterns, responding either by decreased expression or by increased rhythmicity at 15°C compared to 8°C. The results point out mechanisms that can affect the ability of fish to adapt to increasing temperatures caused by climate change.</jats:p>",
"und": "<jats:p>The climate change -driven increase in temperature is occurring rapidly and decreasing the predictability of seasonal rhythms at high latitudes. It is therefore urgent to understand how a change in the relationship between the photoperiod and temperature can affect ectotherms in these environments. We tested whether temperature affects daily rhythms of transcription in a cold-adapted salmonid using high-throughput RNA-sequencing. Arctic char (Salvelinus alpinus) from a subarctic population were reared at a high and a low temperature (15°C and 8°C) for one month under natural, decreasing day length during late summer. Liver transcriptomes were compared between samples collected in the middle and towards the end of the light period and in the middle of the dark period. Daily variation in transcription was lower in fish from the low temperature compared to strong daily variation in warm-acclimated fish, suggesting cold temperatures dampen the cycling of transcriptional rhythms under a simultaneously decreasing day length. Different circadian clock genes had divergent expression patterns, responding either by decreased expression or by increased rhythmicity at 15°C compared to 8°C. The results point out mechanisms that can affect the ability of fish to adapt to increasing temperatures caused by climate change.</jats:p>"
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"en": "Sinha, Vichare, Sinha: Hemispheric comparison of solar flare associated cosmic noise absorption (SCNA) from high latitude stations: Maitri (70.75°S, 11.75°E) and Abisko (68.4°N, 18.9°E)",
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"en": "<jats:p>The effects of solar flares in the D-region ionosphere at two high-latitude stations: Maitri (70.75°S, 11.75°E) and Abisko (68.4°N, 18.9°E), located in different hemispheres are studied. We analyzed 37 M-class flares and 6 X-class flares of the year 2014, which occurred when either or both stations were in the sun-lit side of the Earth. Cosmic Noise Absorption (CNA) curves are obtained using the datasets of riometers located at the two stations and are analyzed for all the 43 events under study. This paper discusses: 1) relationship between CNA and flare magnitude, 2) relationship between CNA and solar zenith angle (SZA), 3) hemispheric asymmetry in the observed solar flare associate CNA (or SCNA), and 4) the effect of background ionospheric condition in the SCNA magnitude at the two high latitude stations. It is observed that the solar flare effect in SCNA strongly depends on the SZA and flare intensity. Our analysis reveals that the flare response in SCNA for the year 2014 was stronger at Abisko than at Maitri. There is an observed hemispheric asymmetry in the solar flare ionization at D-region ionosphere for the given latitude. This asymmetry can be attributed to the previously enhanced background ionospheric ionization during particle precipitation processes. This study shows the need to establish an empirical relationship between the observed CNA vs. flare intensity, SZA and latitudinal position; especially when we go higher in latitudes.</jats:p>",
"fi": "<jats:p>The effects of solar flares in the D-region ionosphere at two high-latitude stations: Maitri (70.75°S, 11.75°E) and Abisko (68.4°N, 18.9°E), located in different hemispheres are studied. We analyzed 37 M-class flares and 6 X-class flares of the year 2014, which occurred when either or both stations were in the sun-lit side of the Earth. Cosmic Noise Absorption (CNA) curves are obtained using the datasets of riometers located at the two stations and are analyzed for all the 43 events under study. This paper discusses: 1) relationship between CNA and flare magnitude, 2) relationship between CNA and solar zenith angle (SZA), 3) hemispheric asymmetry in the observed solar flare associate CNA (or SCNA), and 4) the effect of background ionospheric condition in the SCNA magnitude at the two high latitude stations. It is observed that the solar flare effect in SCNA strongly depends on the SZA and flare intensity. Our analysis reveals that the flare response in SCNA for the year 2014 was stronger at Abisko than at Maitri. There is an observed hemispheric asymmetry in the solar flare ionization at D-region ionosphere for the given latitude. This asymmetry can be attributed to the previously enhanced background ionospheric ionization during particle precipitation processes. This study shows the need to establish an empirical relationship between the observed CNA vs. flare intensity, SZA and latitudinal position; especially when we go higher in latitudes.</jats:p>",
"und": "<jats:p>The effects of solar flares in the D-region ionosphere at two high-latitude stations: Maitri (70.75°S, 11.75°E) and Abisko (68.4°N, 18.9°E), located in different hemispheres are studied. We analyzed 37 M-class flares and 6 X-class flares of the year 2014, which occurred when either or both stations were in the sun-lit side of the Earth. Cosmic Noise Absorption (CNA) curves are obtained using the datasets of riometers located at the two stations and are analyzed for all the 43 events under study. This paper discusses: 1) relationship between CNA and flare magnitude, 2) relationship between CNA and solar zenith angle (SZA), 3) hemispheric asymmetry in the observed solar flare associate CNA (or SCNA), and 4) the effect of background ionospheric condition in the SCNA magnitude at the two high latitude stations. It is observed that the solar flare effect in SCNA strongly depends on the SZA and flare intensity. Our analysis reveals that the flare response in SCNA for the year 2014 was stronger at Abisko than at Maitri. There is an observed hemispheric asymmetry in the solar flare ionization at D-region ionosphere for the given latitude. This asymmetry can be attributed to the previously enhanced background ionospheric ionization during particle precipitation processes. This study shows the need to establish an empirical relationship between the observed CNA vs. flare intensity, SZA and latitudinal position; especially when we go higher in latitudes.</jats:p>"
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"en": "The abundance of juvenile flounder and turbot was surveyed in the VELMU and MARMONI-projects in years 2010-2014 in the Finnish SW-coast at altogether 103 sampling sites, almost all being located on sandy-bottom shores. Surveyes were conducted both on spring (for overwintered 1+ juveniles) and autumn (for young-of-the-year 0+ juveniles). Beach seine was the main survey method, but in year 2010 also a pushnet was tested. As by-catch also information of the occurrence of e.g. cyprinids, gobies and sticklebacks was gathered. Survey data has been used for developing an indicator describing the ecological status of the northern Baltic Sea and it is suitable for coastal spatial planning.\n\n## Method description\nThe abundance of juvenile flounder and turbot was surveyed in the VELMU and MARMONI-projects in years 2010-2014 in the Finnish SW-coast at altogether 103 sampling sites, almost all being located on sandy-bottom shores. Surveyes wereconducted both on spring (for overwintered 1+ juveniles) and autumn (for young-of-the-year 0+ juveniles). Beach seine was the main survey method, but in year 2010 also a pushnet was tested. As by-catch also information of the occurrence of e.g. cyprinids, gobies and sticklebacks was gathered.",
"fi": "Kampela- ja piikkikampelapoikasten esiintymistä kartoitettiin VELMU- ja MARMONI-projekteissa vuosina 2010-2014 yhteensä 103 tutkimusalalla Suomen lounaisrannikolla. \n\nKartoitusaineistoa on käytetty Itämeren tilaa kuvaavan indikaattorin kehitystyössä sekä selvitettäessä kalojen tärkeitä rannikkohabitaatteja. Aineisto sopii taustatiedoksi rannikkoalueiden suunnitteluun. \nAineiston jaellaan karttatasoina (WMS) rajapintapalvelun kautta. Aineiston katseluun suositellaan käytettävän paikkatieto-ohjelmistoa (esim. QGis).\n\nMenetelmäkuvaus:\nKampela- ja piikkikampelapoikasten esiintymistä kartoitettiin VELMU- ja MARMONI-projekteissa 2010-2014 Suomen lounaisrannikolla yhteensä 103 tutkimusalalla. Tutkimusaloiksi valittiin pääasiassa hiekkapohjaisia ranta-alueita, mutta myös sorapohjilta kerättiin näytteitä. Kartoituksia tehtiin sekä keväisin (talvehtineet 1+ juveniilit) että syksyisin (kesänvanhat 0+ poikaset). Kartoitusmenetelmänä käytettiin pääasiassa rantaan vedettävää, pohjassa kulkevaa poikasnuottaa. Vuonna 2010 kokeiltiin kaikilla aloilla myös työntöhaavia (pushnet). Sivusaaliina saatiin tietoja myös mm. särkikalojen, tokkojen ja piikkikalojen poikasvaiheiden esiintymisestä.",
"sv": "Förekomsten av yngel av flundra och piggvar kartlades i projekten VELMU och MARMONI på sammanlagt 103 undersökningsområden längs Finlands sydvästra kust åren 2010–2014. Nästan alla undersökningsområden har sandbotten. Kartläggningar har gjorts både på våren (övervintrade 1+-juveniler) och på hösten (0+-sommaryngel ). Som kartläggningsmetod användes i huvudsak en bottengående yngelnot som dras upp på stranden. År 2010 testades också pushnet-håv på alla undersökningsområden. Som bifångst erhölls information bl.a. om förekomsten av yngelfaser av mörtfiskar, smörbultar och spiggartade fiskar. Kartläggningsmaterialet har använts för utveckling av en indikator som beskriver Östersjöns tillstånd och för utredning av kusthabitat som är viktiga för fiskar. Materialet lämpar sig som bakgrundsinformation vid planering av kustområden."
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