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Clusters were defined based on the expression of canonical markers and known cell surface expression of different CD45 + cell types. b The expression of canonical markers of different cell types in each cluster as identified in UMAP in 1b. c Proportion of cells from individual patients in each cluster. d Absolute number of cells from individual patients in each cluster. e UMAP representation of the IMA dataset split by individual patients. Figure S3. Phenotypic characterization of CD45 + cells in the cohort (cont.). a Feature plot of inflammation (LMNA, CREM) and activation (CXCL13, CCL5, CCL4 and RGCC) associated genes identified in the UMAP 1b. b Activation, inflammation, exhaustion, and inhibitory module scores as compared between tissues. c Activation, inflammation, exhaustion, and inhibitory module scores as compared between cells belonging to expanded versus not expanded clones. d Clonality index (Gini, higher Gini denotes more clonal) between ST and PB. e Clonality index (Gini) between ST and PB in individual samples. Figure S4. a Phenotypic characterization of CD4 + T cells in the cohort. Proportion of the cells from ST and PB in each of the CD4 + T cell clusters shown in Fig. 2b. b Relative proportion of the cells from ST and PB in each cluster as identified in the UMAP in Fig. 2b, each cluster represents 100% of the cell population. c Left: Proportion of the cells from individual patients as identified in the UMAP in Fig. 2b. Right: Proportion of the cells from individual samples as identified in the UMAP in Fig. 2b. d Expression of phenotypic markers in CD4 + T cell clusters. e Expression of phenotypic markers as identified in the UMAP 2b. f Clonal overlap (as measured by TCR similarity by Morisita index) between clusters as identified in the UMAP 2b. g Proportion of cells in different phases of cell cycle in each cluster as identified in the UMAP 2b in all CD4 + T cells in ST and PB. h The expression of proliferation-associated transcripts G0S2, FABP5, and MKI67 in ST compared to PB. (Bonferroni corrected two-sided t-test). Figure S5. a Phenotypic characterization of CD4 + cells in the cohort (cont.). Proliferation score (based on the expression of proliferation associated genes) of Expanded versus Non-expanded cells. b Samples were merged with scVI with tissue of origin as the batch key, to reduce batch effect. Left: UMAP representation and unsupervised clustering of the cells. Right: UMAP representation of the cells, coloured according to tissue origin. c UMAP representation of all cells from the Wu et al. cohort (Wu et al. 2021), coloured according to unsupervised clustering (left) and tissue origin (right). d A proliferation score (based on the expression of proliferation associated genes) of synovial membrane versus peripheral blood in the Wu et al. cohort (Wu et al. 2021). Figure S6. a Phenotypic characterization of CD8 + cells in the cohort. Proportion of the cells from ST and PB in each of the clusters shown in Fig. 2f. b Relative proportion of the cells from ST and PB in each cluster as identified in the UMAP in Fig. 2f, each cluster represents 100% of the cell population. c Left: Proportion of the cells from individual patients as identified in the UMAP in Fig. 2f. Right: Proportion of the cells from individual samples as identified in the UMAP in Fig. 2f. d Proportion of cells in different phases of cell cycle. e The expression of phenotypic markers in the clusters shown in Fig. 2f. Figure S7. a Clonal trafficking of CD4 + and CD8 + T cells between PB and ST. UMAP representation of all cells with intersecting clones between ST and PB as identified in the UMAP 2b. b UMAP representation of all cells with intersecting clones split by original patient between ST and PB as identified in the UMAP 2b. c Proportion of intersecting clones between ST and PB in different clusters as identified in the UMAP 2b. d Proportion of intersecting clones between ST and PB in different tissue of origin as identified in the UMAP 2b. e Antigen-specificities of the TCR repertoire from CD4 + cells matched against VDJdb. The most common target species have been highlighted. f Proportion of cells in different clusters identified with a unifying motif as predicted by GLIPH2. g Proportion of intersecting clones between ST and PB in different clusters as identified in the UMAP 2f. h Proportion of intersecting clones between ST and PB in different tissue of origin as identified in the UMAP 2f."
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