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Specifications Model: Pix2Pix for generating synthetic nuclear staining from brightfield images of circulating immune cells Training Dataset: Immune Cells: 226 paired brightfield and fluorescent microscopy images Microscope: Nikon Eclipse Ti2-E, brightfield/fluorescence microscope with a 20x objective Data Type: Brightfield and fluorescent microscopy images File Format: TIFF (.tif), 16-bit Image Size: 1024 x 1022 pixels (Pixel size: 650 nm) Training Parameters: Epochs: 400 Patch Size: 512 x 512 pixels Batch Size: 1 Loss Function: Vanilla GAN loss function Model Performance: Immune Cells: SSIM Score: 0.756 LPIPS Score: 0.130 Model Selection: Models were chosen based on quality metric scores and visual inspection compared to ground truth images. 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